Yes, then you need to handle FlyTreeView.NodeDataBound event and then
string val1 = DataBinder.Eval(e.Node.DataItem, \"YourColumn1\");
string val2 = DataBinder.Eval(e.Node.DataItem, \"YourColumn2\");
e.Node.Text = string.Format(\"{0}: {1}\", val1, val2);
Or something like this. I didn't test the code.